| Comparative Coagulation |
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Sample Submission, Collection
and Processing
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The accuracy and validity of hemostasis testing
depends on sample quality!
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Technique for collection of whole blood in citrate anticoagulant: |
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DOGS, CATS, PRIMATES, RABBITS |
Syringe Method:
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- Draw citrate anticoagulant (3.8% Na citrate) into a plastic
syringe and then perform venipuncture using a ratio of 1 part
citrate: 9 parts blood as follows:
0.3 mL citrate plus 2.7 mL blood = 3.0 mL total sample
0.4 mL citrate plus 3.6 mL blood = 4.0 mL total sample
0.5 mL citrate plus 4.5 mL blood = 5.0 mL total sample
(CITRATE TO WHOLE BLOOD RATIO IS CRITICAL)
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Vacutainer Method:
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- Use a vacutainer needle or vacutainer butterfly set to draw
blood directly into a 3 or 5 ml sodium citrate vacutainer tube.
A COMPLETE DRAW MUST BE OBTAINED.
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LARGE ANIMALS (COW, HORSE, SHEEP, GOAT) |
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Use a vacutainer needle and draw blood directly into a 5 - 10
mL blue top (3.8% Na citrate) vacutainer tube. A COMPLETE DRAW
MUST BE OBTAINED.
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LABORATORY ANIMALS (RODENTS, SMALL MAMMALS): |
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(Do not use the eye orbital vein or tail vein collection technique)
Anesthesia (deep surgical plane) is necessary for blood collection.
Suitable anesthetic agents include pentobarbital or ketamine/xylazine.
Incise the body wall and peritoneum on the ventral midline in
order to expose the abdominal aorta for needle puncture.
Place citrate anticoagulant (3.8% Na citrate) into a plastic syringe
and then draw blood using a 22 or 23 gauge needle. The ratio of
citrate to blood must be exactly 1: 9 as in the following examples:
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- 0.1 mL citrate plus 0.9 mL blood = 1.0 mL total sample
0.2 mL citrate plus 1.8 mL blood = 2.0 mL total sample
0.3 mL citrate plus 2.7 mL blood = 3.0 mL total sample
(CITRATE TO WHOLE BLOOD RATIO IS CRITICAL)
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Plasma separation and storage: |
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Centrifuge whole blood in a plastic or siliconized glass tube for
1 min. in a microfuge or for 10 min. in a tabletop centrifuge.
Transfer PLASMA ONLY into a plastic tube using a plastic pipet
Store samples frozen (-70oC to -20oC) until shipment |
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Shipping requirements: |
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Ship samples in a styrofoam box with cold packs.
Ship overnight to arrive next day morning or afternoon
Wrap tubes to prevent breakage.
Dry ice (3-5 lbs) may be required for shipment of special studies
(call for details).
Complete a Animal Health Diagnostic
Center submission form
Contact information for low
cost courier service |
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Shipping address: |
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Comparative Coagulation Section-Animal Health Diagnostic Center
College of Veterinary Medicine, Cornell University |
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(address for US Mail)
P.O. Box 5786
Upper Tower Road
Ithaca, NY 14852-5786 |
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(address for courier service)
Upper Tower Road
Ithaca, NY 14853 |
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Cornell CVM or on-campus submissions: |
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Citrate whole blood samples can be dropped off at the Diagnostic Center
receiving window for processing from 8:00 AM to 5:00 PM |
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After hours |
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Store separated citrate plasma in the refrigerator (no longer than
overnight - 18 hours). Transport to the Diagnostic Center first
thing in the morning.
Note - Platelet function testing requires 24 hours notice and specific
arrangement with the Coagulation Section (call 275-0622). Samples
must remain at room temperature until transport to the Coagulation
Section. |
