Cornell University home pageCollege of Veterinary Medicine home page
Population Medicine & Diagnostic Sciences home page
Education    Research    Services    Departments    News

 
Clinical Pathology Laboratory - Available Test
Centesis Fluid Cytology Report
 
 
An example of our centesis fluid report is indicated below. For all centesis fluids, we perform the following:
Visual assessment: Color, turbidity, volume.
Cell counts: This includes both red and white blood cell counts. This is done manually using our bench top impedance counter, the Coulter Z2. Results are reported out as thousands of cells/µL.
Total protein: This is measured by refractometer.
Cytologic evaluation: The type of smear made depends on the cellularity of the fluid.
 

Photomicrograph of a peritoneal effusion in a dog with pancreatitis. There is a sterile peritonitis characterized by large numbers of non-degenerate neutrophils (black arrows) with fewer leukophagocytic macrophages (red arrowheads).
 
Centesis fluids are generally stable for up to 24 hours after collection, if refrigerated. Some changes will occur in vitro, such as phagocytosis of erythrocytes and bacteria. This complicates result interpretation, so freshly made smears (direct or sediment) should be provided with the fluid when sample submission is delayed. If this is done, please specify the type of smear made, i.e. direct or sediment, on the request form. If a sediment smear is made, leave at least 1 ml of fluid uncentrifuged, in order to allow us to perform accurate cell counts.