Method of Urinalysis
The routine urinalysis is a quick and relatively inexpensive test which can be readily
performed in a modest clinical laboratory. The results are useful in a variety of
situations not limited to those directly involving the urinary tract.
Required equipment and materials include: microscope, clinical centrifuge, refractometer,
and multiple reagent dipsticks. An outline of the procedure:
- Refrigerated urine should always be brought to room temperature before testing.
- Observations of color and turbidity are made on the well-mixed urine specimen.
- The multireagent dipstick should be used in well-mixed "whole" urine. Follow manufacturer's
directions on storage and use of the reagents. The package insert also contains useful information
about test limitations and interfering substances.
- The specific gravity should be read on the refractometer using the urine supernatant.
- The sediment is prepared by resuspending the pellet in a small amount of urine. A drop of the
suspension is examined microscopically as an unstained, coverslipped wet-mount. Subdued lighting
is necessary to increase refractility of the unstained urine elements (lower condenser and / or
close down the substage iris diaphragm).
|